mouse il-11 duoset elisa Search Results


mouse il11 duoset elisa  (R&D Systems)
94
R&D Systems mouse il11 duoset elisa
Figure 2. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific gp130 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of APAP induction in wild-type (WT) and CKOgp130 mice: overnight-fasted mice were injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h post-APAP dosing for experiments shown in (C–F) and Figure 3. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of <t>IL11,</t> NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, p-STAT3, STAT3, Cl. CASP3, and CASP3 (n = 3/group) at 6 h post-APAP. (G) Representa- tive H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing and quantification of liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box) and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).
Mouse Il11 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 11 duoset elisa kit  (R&D Systems)
94
R&D Systems mouse il 11 duoset elisa kit
Figure 2. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific gp130 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of APAP induction in wild-type (WT) and CKOgp130 mice: overnight-fasted mice were injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h post-APAP dosing for experiments shown in (C–F) and Figure 3. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of <t>IL11,</t> NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, p-STAT3, STAT3, Cl. CASP3, and CASP3 (n = 3/group) at 6 h post-APAP. (G) Representa- tive H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing and quantification of liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box) and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).
Mouse Il 11 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+il-11+duoset+elisa/Mouse+IL-11+DuoSet+ELISA/bio_rxiv__336537-163-15-21
Average 94 stars, based on 1 article reviews
mouse il 11 duoset elisa kit - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


Figure 2. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific gp130 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of APAP induction in wild-type (WT) and CKOgp130 mice: overnight-fasted mice were injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h post-APAP dosing for experiments shown in (C–F) and Figure 3. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of IL11, NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, p-STAT3, STAT3, Cl. CASP3, and CASP3 (n = 3/group) at 6 h post-APAP. (G) Representa- tive H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing and quantification of liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box) and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).

Journal: International journal of molecular sciences

Article Title: Hepatocyte Specific gp130 Signalling Underlies APAP Induced Liver Injury.

doi: 10.3390/ijms23137089

Figure Lengend Snippet: Figure 2. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific gp130 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of APAP induction in wild-type (WT) and CKOgp130 mice: overnight-fasted mice were injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h post-APAP dosing for experiments shown in (C–F) and Figure 3. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of IL11, NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, p-STAT3, STAT3, Cl. CASP3, and CASP3 (n = 3/group) at 6 h post-APAP. (G) Representa- tive H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing and quantification of liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box) and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) and Colorimetric Assays The levels of IL11 in mouse serum were quantified using Mouse IL11 DuoSet ELISA (DY418, R&D Systems, Minneapolis, MN, USA).

Techniques: Control, Immunohistochemistry, Injection, Saline, Western Blot, Staining, Whisker Assay

Figure 3. Hepatocyte-specific deletion of gp130 limits APAP-induced liver injury and promotes hepatic regeneration 24 h after injury. (A) Serum IL11 levels, (B) serum ALT levels, (C) serum AST levels, (D) hepatic GSH levels, (E) Western blots showing hepatic levels of IL11, PCNA, Cyclin D1, and GAPDH as internal contro (n = 3/group)l, (F) hepatic mRNA expression of pro-inflammatory markers (Ccl2, Ccl5, Il1β, Il6, Tnfα), (G) Western blots showing hepatic levels of p-ERK, ERK, p-JNK, JNK, Cl. CASP3, CASP3, p-STAT3, and STAT3 (n = 3/group), (H) representative H&E-stained liver images (scale bars, 200 µm) and quantification of liver necrotic area, (I) IHC staining of Ki67 in the livers of mice (scale bars, 200 µm) and quantification of Ki67+ve cells for experiments shown in Figure 2B. (A–D,F,H,I) Saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype); data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction.

Journal: International journal of molecular sciences

Article Title: Hepatocyte Specific gp130 Signalling Underlies APAP Induced Liver Injury.

doi: 10.3390/ijms23137089

Figure Lengend Snippet: Figure 3. Hepatocyte-specific deletion of gp130 limits APAP-induced liver injury and promotes hepatic regeneration 24 h after injury. (A) Serum IL11 levels, (B) serum ALT levels, (C) serum AST levels, (D) hepatic GSH levels, (E) Western blots showing hepatic levels of IL11, PCNA, Cyclin D1, and GAPDH as internal contro (n = 3/group)l, (F) hepatic mRNA expression of pro-inflammatory markers (Ccl2, Ccl5, Il1β, Il6, Tnfα), (G) Western blots showing hepatic levels of p-ERK, ERK, p-JNK, JNK, Cl. CASP3, CASP3, p-STAT3, and STAT3 (n = 3/group), (H) representative H&E-stained liver images (scale bars, 200 µm) and quantification of liver necrotic area, (I) IHC staining of Ki67 in the livers of mice (scale bars, 200 µm) and quantification of Ki67+ve cells for experiments shown in Figure 2B. (A–D,F,H,I) Saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype); data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction.

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) and Colorimetric Assays The levels of IL11 in mouse serum were quantified using Mouse IL11 DuoSet ELISA (DY418, R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Expressing, Staining, Immunohistochemistry, Saline, Whisker Assay

Figure 4. Generation and validation of hepatocyte-specific Il11-deficient adult mice. (A) Schematic design showing the deletion of exons 2–5 of the mouse Il11 locus and the gene targeting strategy. (B) Representative genotyping of Il11+/+, Il11loxP/+, or Il11loxP/loxP mice. (C) Schematic showing AAV8-induced hepatocyte-specific Il11 knockout mice (CKOIl11). (D) Il11 mRNA (n = 5/group) and (E) IL11 protein (n = 3/group) expression in the heart, liver, lung, and kidney from WT and CKOIl11

Journal: International journal of molecular sciences

Article Title: Hepatocyte Specific gp130 Signalling Underlies APAP Induced Liver Injury.

doi: 10.3390/ijms23137089

Figure Lengend Snippet: Figure 4. Generation and validation of hepatocyte-specific Il11-deficient adult mice. (A) Schematic design showing the deletion of exons 2–5 of the mouse Il11 locus and the gene targeting strategy. (B) Representative genotyping of Il11+/+, Il11loxP/+, or Il11loxP/loxP mice. (C) Schematic showing AAV8-induced hepatocyte-specific Il11 knockout mice (CKOIl11). (D) Il11 mRNA (n = 5/group) and (E) IL11 protein (n = 3/group) expression in the heart, liver, lung, and kidney from WT and CKOIl11

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) and Colorimetric Assays The levels of IL11 in mouse serum were quantified using Mouse IL11 DuoSet ELISA (DY418, R&D Systems, Minneapolis, MN, USA).

Techniques: Biomarker Discovery, Knock-Out, Expressing

Figure 5. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific Il11 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of induction of APAP injury in wild-type (WT) and CKOIl11 mice: overnight-fasted mice were intraperitoneally injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h following APAP administration for experiments shown in (C–F) and Figure 6. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of IL11, NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, Cl. CASP3, and CASP3 at 6 h post-APAP. (G) Representative H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing quan- tification of the liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6 h time point: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).

Journal: International journal of molecular sciences

Article Title: Hepatocyte Specific gp130 Signalling Underlies APAP Induced Liver Injury.

doi: 10.3390/ijms23137089

Figure Lengend Snippet: Figure 5. Hepatic CYP2E1 and glutathione levels and serum and molecular markers of liver injury in mice with hepatocyte-specific Il11 deletion and control mice. (A) Representative immunohisto- chemistry images (scale bars, 200 µm; representative dataset from n = 3 mice/group). (B) Schematic of induction of APAP injury in wild-type (WT) and CKOIl11 mice: overnight-fasted mice were intraperitoneally injected with saline or APAP (400 mg kg−1); livers and serum were collected either 0.5, 6, or 24 h following APAP administration for experiments shown in (C–F) and Figure 6. (C) Hepatic GSH concentrations at 0.5 and 6 h post-APAP. (D) ALT, (E) AST, and (F) Western blots of IL11, NOX4, GAPDH, p-ERK, ERK, p-JNK, JNK, Cl. CASP3, and CASP3 at 6 h post-APAP. (G) Representative H&E-stained liver images (scale bars, 200 µm) at 6 h post-APAP dosing quan- tification of the liver necrotic area. (C–E,G) Data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction; 0.5 h time point: saline (n = 4 mice/genotype), APAP (n = 4 mice/genotype), 6 h time point: saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype).

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) and Colorimetric Assays The levels of IL11 in mouse serum were quantified using Mouse IL11 DuoSet ELISA (DY418, R&D Systems, Minneapolis, MN, USA).

Techniques: Control, Immunohistochemistry, Injection, Saline, Western Blot, Staining, Whisker Assay

Figure 6. Hepatocyte-specific deletion of Il11 reduces APAP-induced liver injury and promotes hepatic regeneration 24 h after injury. (A) Serum IL11 levels, (B) Western blots showing hepatic levels of IL11, PCNA, Cyclin D1, and GAPDH as the internal control (n = 3/group), (C) serum ALT levels, (D) serum AST levels, (E) hepatic GSH levels, (F) hepatic mRNA expression of pro-inflammatory markers (Ccl2, Ccl5, Il1β, Il6, Tnfα), (G) Western blots showing hepatic levels of p-ERK, ERK, p-JNK, JNK, Cl. CASP3, CASP3, p-STAT3, and STAT3 (n = 3/group), (H) representative H&E-stained liver images (scale bars, 200 µm) and quantification of the liver necrotic area, (I) IHC staining of Ki67 in the livers of mice (scale bars, 200 µm) and quantification of Ki67+ve cells for experiments shown in Figure 5B. (A,C–F,H,I) Saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype); data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction.

Journal: International journal of molecular sciences

Article Title: Hepatocyte Specific gp130 Signalling Underlies APAP Induced Liver Injury.

doi: 10.3390/ijms23137089

Figure Lengend Snippet: Figure 6. Hepatocyte-specific deletion of Il11 reduces APAP-induced liver injury and promotes hepatic regeneration 24 h after injury. (A) Serum IL11 levels, (B) Western blots showing hepatic levels of IL11, PCNA, Cyclin D1, and GAPDH as the internal control (n = 3/group), (C) serum ALT levels, (D) serum AST levels, (E) hepatic GSH levels, (F) hepatic mRNA expression of pro-inflammatory markers (Ccl2, Ccl5, Il1β, Il6, Tnfα), (G) Western blots showing hepatic levels of p-ERK, ERK, p-JNK, JNK, Cl. CASP3, CASP3, p-STAT3, and STAT3 (n = 3/group), (H) representative H&E-stained liver images (scale bars, 200 µm) and quantification of the liver necrotic area, (I) IHC staining of Ki67 in the livers of mice (scale bars, 200 µm) and quantification of Ki67+ve cells for experiments shown in Figure 5B. (A,C–F,H,I) Saline (n = 5 mice/genotype), APAP (n = 6 mice/genotype); data are shown in a box-and-whisker plot with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), with a 2-way ANOVA with Sidak’s correction.

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) and Colorimetric Assays The levels of IL11 in mouse serum were quantified using Mouse IL11 DuoSet ELISA (DY418, R&D Systems, Minneapolis, MN, USA).

Techniques: Western Blot, Control, Expressing, Staining, Immunohistochemistry, Saline, Whisker Assay